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grp94  (Wuhan Sanying Biotechnology)
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Wuhan Sanying Biotechnology grp94
Effects of different concentrations of melatonin on ER stress and autophagy in N2a neuroblastoma cells. (A) The model of Pak2 overexpression and expression inhibition was constructed, and western blotting was used to detect the effects of different concentrations of melatonin on ER stress in neuroblastoma cells. (B-D) The expression of <t>GRP94,</t> GRP78 and CHOP proteins increased compared with the Pak2 overexpression and Pak2 inhibition groups with the same concentration of melatonin. (E) For the groups of AMPK signaling pathway inhibitors and DMSO solvent at the same melatonin concentration, the protein levels of <t>GRP94,</t> GRP78 and CHOP were detected via Western blot analysis. Compared with the AMPK signaling pathway inhibitor and DMSO solvent groups with the same concentration of melatonin, the expression of (F) CHOP, (G) GRP78 and (H) GRP94 proteins was weakened (F-H). **P<0.01, ***P<0.001, ****P<0.0001. ER, endoplasmic reticulum; N2a, Neuro-2a; Pak2, p21-activated kinase 2; GRP, glucose-regulated protein; AMPK, 5′-AMP-activated protein kinase; Mel, melatonin; OE, Pak2 overexpression; KD, Pak2 knockdown; Dorso, dorsomorphin; DMSO, solvent control group.
Grp94, supplied by Wuhan Sanying Biotechnology, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Effects of different concentrations of melatonin on ER stress and autophagy in N2a neuroblastoma cells. (A) The model of Pak2 overexpression and expression inhibition was constructed, and western blotting was used to detect the effects of different concentrations of melatonin on ER stress in neuroblastoma cells. (B-D) The expression of GRP94, GRP78 and CHOP proteins increased compared with the Pak2 overexpression and Pak2 inhibition groups with the same concentration of melatonin. (E) For the groups of AMPK signaling pathway inhibitors and DMSO solvent at the same melatonin concentration, the protein levels of GRP94, GRP78 and CHOP were detected via Western blot analysis. Compared with the AMPK signaling pathway inhibitor and DMSO solvent groups with the same concentration of melatonin, the expression of (F) CHOP, (G) GRP78 and (H) GRP94 proteins was weakened (F-H). **P<0.01, ***P<0.001, ****P<0.0001. ER, endoplasmic reticulum; N2a, Neuro-2a; Pak2, p21-activated kinase 2; GRP, glucose-regulated protein; AMPK, 5′-AMP-activated protein kinase; Mel, melatonin; OE, Pak2 overexpression; KD, Pak2 knockdown; Dorso, dorsomorphin; DMSO, solvent control group.

Journal: Molecular Medicine Reports

Article Title: Melatonin induces autophagy in neuroblastoma by alleviating Pak2-mediated endoplasmic reticulum stress

doi: 10.3892/mmr.2025.13784

Figure Lengend Snippet: Effects of different concentrations of melatonin on ER stress and autophagy in N2a neuroblastoma cells. (A) The model of Pak2 overexpression and expression inhibition was constructed, and western blotting was used to detect the effects of different concentrations of melatonin on ER stress in neuroblastoma cells. (B-D) The expression of GRP94, GRP78 and CHOP proteins increased compared with the Pak2 overexpression and Pak2 inhibition groups with the same concentration of melatonin. (E) For the groups of AMPK signaling pathway inhibitors and DMSO solvent at the same melatonin concentration, the protein levels of GRP94, GRP78 and CHOP were detected via Western blot analysis. Compared with the AMPK signaling pathway inhibitor and DMSO solvent groups with the same concentration of melatonin, the expression of (F) CHOP, (G) GRP78 and (H) GRP94 proteins was weakened (F-H). **P<0.01, ***P<0.001, ****P<0.0001. ER, endoplasmic reticulum; N2a, Neuro-2a; Pak2, p21-activated kinase 2; GRP, glucose-regulated protein; AMPK, 5′-AMP-activated protein kinase; Mel, melatonin; OE, Pak2 overexpression; KD, Pak2 knockdown; Dorso, dorsomorphin; DMSO, solvent control group.

Article Snippet: The antibodies used in this experiment were as follows: Rabbit resist grp94 (1:8,000; cat. no. 14700-1-AP; Wuhan Sanying Biotechnology), CHOP (1:20,000; cat. no. 15204-1-AP; Wuhan Sanying Biotechnology), LC3 (1:20,000; cat. no. 14600-1-AP; Wuhan Sanying Biotechnology), ATG5 (1:5,000; cat. no. 10181-2-AP; Wuhan Sanying Biotechnology), Beclin1 (1:5,000; cat. no. bs-1353R; BIOSS), P62(1:5,000; cat. no. 18420-1-AP; Wuhan Sanying Biotechnology), PAK2 (1:10,000; cat. no. 21401-1-AP; Wuhan Sanying Biotechnology), ULK1 (1:10,000; cat. no. 68445-1-Ig; Wuhan Sanying Biotechnology), p-ULK1(1:50,000; cat. no. 80218-1-RR; Wuhan Sanying Biotechnology), mouse resist grp78 (1:10,000; cat. no. GB15098; Wuhan Servicebio Technology Co., Ltd), mTOR (1:10,000; cat. no. 66888-1-Ig; Wuhan Sanying Biotechnology) and p-mTOR (1:5,000; cat. no. 67778-1-Ig; Wuhan Sanying Biotechnology).

Techniques: Over Expression, Expressing, Inhibition, Construct, Western Blot, Concentration Assay, Solvent, Knockdown, Control